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Stimulus duration was 100 µs, and half maximal stimulus intensity was used (less than 800 µA, usually 200 800 µA for C-fibers).
Test stimulus was set at 50% maximal stimulus intensity to evoke optical EPSP (op-EPSP) in each slice.
The half maximal stimulus intensity was used to evoke EPSCs at holding potentials between −90 and +50 mV.
Input-output curves of the excitatory postsynaptic currents (EPSCs) were evoked at a holding potential of −70 mV to determine the half maximal stimulus intensity.
Once the maximal stimulus intensity and optimal muscle length (Lo) for force production were determined (at 50 Hz and train of 500 milliseconds), peak twitch tension (Ptw), time-to-peak twitch tension (TPT) and RT½ (the time for Ptw to relax to one-half Ptw) were determined at Lo from a series of contractions induced by single-pulse stimuli.
Similar(55)
Although this is a possibility, we did not see a change in the size of eIPSCs following PMA activation, unlike previous studies that demonstrated increases with the phorbol ester, phorbol dibutyrate, in guinea pig VTA (Bonci and Williams, 1997), although our observed lack of eIPSC amplitude increase could be because we stimulated at near maximal stimulus intensities.
PPI consisted of eliciting equivalent paired pulses 20 ms apart at half-maximal stimulus intensity [39].
Strong inhibition produced by delivery of stimuli at maximal stimulus strength (100% maximum) revealed little difference among the groups.
After the hotspot of the ECR was located, RMT and the active motor threshold (AMT) were determined, defined as the minimal stimulus intensity necessary to produce MEPs larger than 0.1 mV in at least five out of ten consecutive trials while subjects maintained a slight voluntary contraction of the ECR at approx. 3% of the maximal voluntary contraction.
An I O curve was constructed by plotting the relative amplitude of eIPSCs to the maximal amplitude at each stimulus intensity.
Threshold for eliciting MEP was defined as the lowest stimulus intensity (% of maximal stimulator output) capable of eliciting at least five MEP with an amplitude of at least 100 μV in a relaxed hand muscle in a series of ten consecutive trials of TMS [27].
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