Normal mouse islets do not secrete insulin when exposed to 3 mM glucose, whereas half-maximal stimulation is achieved at ~11 mM glucose (Hedeskov, 1980).
In 5-day MMC, 130% stimulation of PG synthesis occurred at 2.5 μg/ml PPS (P < 0.0001), while 5.0 μg/ml PPS achieved maximal stimulation in the 7-day and 10-day cultures (P < 0.05).
Maximal stimulation of LTED cells was achieved at 10-14 mol/l, while stimulation of wild-type MCF-7 cells was maximal at 10-10 mol/l E2.
Yet, maximal stimulation of the reconstituted transporter was achieved at lower drug concentrations, suggesting that its substrate affinity and/or coupling mechanism between transmembrane and nucleotide-binding domains were favored upon reconstitution.
Shaw and colleagues [ 28] showed, when providing 1.5 g/kg per day protein to severely septic patients, that maximal stimulation of protein synthesis could be achieved.
In mERlow cells the response peak was achieved with a 50-fold lower E2 concentration; the higher maximal stimulation of cAMP production in mERhigh cells was achieved with 100 pmol/l E2.
In the T47D proliferation assay, maximal stimulation (2.2-fold over basal levels, reaching a plateau) was achieved with 100 ng/ml of PRL treatment [ 29].
Maximal stimulation of PKC α translocation in intact nonirradiated acinar cells from the parotid gland was achieved with 3 min incubation with 1 μ M PMA (data not shown), and 3 min incubation time with PMA lead to a dose-dependent increase of PKC α in the cell membrane fraction.
Inosine stimulated protein kinase activity at low concentration, and adenosine showed maximal stimulation at 0.1 mM.
In 5-day MMC, maximal stimulation (130%) of PG synthesis occurred at 2.5 μg/ml PPS (P < 0.0001), while 5.0 μg/ml PPS was required to achieve maximal stimulation (160%, P < 0.01) on day 7. Stimulation (150%, P < 0.05) was also observed, however, with the lower concentration of 2.5 μg/ml PPS in this time period.
