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[68Ga]NOTA-pentixafor was obtained with radiochemical yields of 86.6 ± 3.1 % and a maximal specific activity of 128 GBq/μmol.
Under the favorable fermentation conditions, the maximal specific activity of BphC_LA-4 was about 0.58 U/mg with catechol as substrate.
Additionally, the obtained specific activities are much higher than of the purified d-Hyd(co) with a maximal specific activity of 20.1 mU/mg, which is probably caused by a higher stability of the d-Hyd(co) upon immobilization.
177LuCl3 with 740 MBq/μg maximal specific activity was obtained from Perkin Elmer (St . Louis MO, USA).177LuCl3 was diluted to 4.2-5.3 MBq/μl and incubated with the DOTA-Tyr3-octreotate solution for 30 min at 80°C.
For thermodynamic experiments, we used the enzyme that has >90% of its maximal specific activity.
This maximal specific activity of each LAAO is related to the optimum pH for each type of amino acid acting as the substrate [ 58].
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Figure 2 Maximal specific activities.
Maximal specific activities (in megabecquerel per nanomole) for DTPA-exendin-3, DOTA-exendin-3, DTPA-octreotide, DOTA-octreotide, DTPA-anti-CAIX and DOTA-anti-CAIX in 0.1 M NaAc, NH4Ac, MES and HEPES buffers.
The aim of the study is to derive conditional clearance levels (maximal specific mass activity of material allowing its recycling/clearance) for analyzed radionuclides ensuring that the detrimental impact on human health is kept on a negligible level.
In addition, a 4 6 concentration ratio of PDCzm-slr1192 would endow the reaction with a maximal specific catalytic activity.
Using this standard assay, the maximal measured specific activity was about 30 mU per mg protein in cell extract.
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