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MS/MS identifications were transferred between LC-MS/MS runs with the 'Match between runs' option, in which the maximal retention time window was set to 2 min. For every peptide, corresponding total signals from multiple runs were compared to determine peptide ratios.
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Seed retention times were considerably long, with peak defecation at 48 72 hours after ingestion and maximal retention times of up to 670 hours (Figure 1).
The outputs of the supervised analysis were exported as Cmpd Mass Spec List Report MS (P) Layout and contained information on the retention time, maximal intensity, and area after smoothing.
Peak 1 occurs at retention time of 19.6 min with maximal UV absorption at 319 nm.
Peak 2 shows the retention time of 45.1 min with maximal UV absorption at 281 nm.
Peak 7 was eluted at retention time of 73.8 min with maximal UV absorption at 223 and 286 nm.
Peak 6 was eluted at retention time of 70.1 min with maximal UV absorption at 222 and 288 nm, which produced the [M-H]- ion at 269 in the MS spectrum in negative ion mode.
tR - retention time; λmax - maximum observed absorbance.
The increasing of hydraulic retention time and the improvement of design for maximal contact between material and wastewater are considered to be key factors that can provide optimal pH for the removal processes.
An unidentified peak (retention time of 15.2 min) was detected with a maximal area at day 7 and decreased until the end of the experiment.
During controlled laboratory exposures of striped mullet to K. brevis, blood brevetoxin concentrations varied according to the K. brevis/brevetoxin exposure concentrations where maximal blood brevetoxin concentrations were ∼19 ng/mL at 8 to 12 hr post-exposure with a protracted retention time (T1/2) of over 11 days [66].
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