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Magnitude of STD was defined as maximal responses within the first minutes after induction by a train of 900 stimuli (1 Hz).
The magnitude of STP was defined as the maximal responses within the first minutes after induction by a train of 100 Hz stimuli.
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Simultaneously, as the pigment increased to a maximal response within 7 10 days (Fig. 2A), hairs sprouted out and grew to full length with 95 100% of hair coverage in 2 weeks (Figs. 1C and 2B).
In accordance with that, the GR/cortisol-dependent expression profiles of nuclear receptor genes show an early maximal response within the first 24 h, followed by a lower level of repression at later time points.
There was a significant increase within 2-4 h, reached a maximal response within 6 h, and declined within 24 h.
There was a significant increase in COX-2 mRNA within 30 min, and reached a maximal response within 2 h.
It is clear that at both voltages ACh application led to a maximal response within seconds of its application.
The results showed that ET-1 time-dependently stimulated PGE2 release and a significant PGE2 production was observed within 4 h, reached a maximal response within 6 h and slightly declined within 24 h.
As shown in Figure 6A, ET-1 enhanced NF-κB transcriptional activity in a time-dependent manner with a maximal response within 60 min, which was significantly inhibited by pretreatment with an inhibitor of NF-κB (Bay11-7082, 10 nM).
As shown in Figure 5C, BK stimulated PKC-α translocation in a time-dependent manner with a maximal response within 5 min, which was attenuated by pretreatment with either BAPTA/AM (30 μM) or Gö6976 (1 μM).
At both voltages ET-1 application led to a maximal response within seconds of its application (mean I– V data for peak ET-1 activated current are shown in Fig. 3B, showing the response to be very similar to that to ACh (Fig. 1B), though of smaller magnitude).
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