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Strikingly, higher concentrations of mibolerone were able to induce maximal responses between 85 105% of that observed with the WT receptor (EC50 range = 2.6 71.6 nM).
Dacarbazine induced minimal, albeit dose-dependent apoptosis, with maximal responses between 10 25%.
Time-course analysis of two FLS lines revealed maximal responses between 8 and 24 hr for TNF-α (10 ng/ml) and 8 and 16 hr for corticosterone (100 nmol/l) (Additional file 3).
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On the other hand, there was a clear difference in the pH values eliciting the half maximal response between WT (pH 7.1) and H11Y(B) (pH 6.5) (Fig. 4C).
In our studies, the CT response to N + Bz + GalA-MRP gave the maximal response between pH 6 and 7.
A dose dependent increase in ankle diameter was observed in the 2.5 μg, 5 μg and 10 μg SCW treated groups (Δ0.65, Δ2.7, Δ3 mm, respectively) compared to the non-injected controls with a maximal response between days 24 25.
For R1881, the maximal responses ranged between 40 60% of that observed with the WT AR, but occurred at higher concentrations (EC50 range = 10.6 159.4 nM).
There was no significant difference in half-maximal response between both groups.
Data for each variant were fitted with a Boltzmann equation, yielding half maximal responses (V1/2 values) between at +60 to +100 mV (Fig. 2D).
When IP accumulation was measured in sensory neuron cultures after treatment with ET-1 or BK, significant differences between maximal responses of the agonists were observed).
The maximal responses of the tested compounds ranged between a 3.2-fold increase and a 7.7-fold increase (T – 6.4, DHT – 7.7, Mb – 7.1, R1881 – 3.2).
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