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The findings reveal that after the simultaneous dual optimization, the maximal response values were obtained at an activation temperature of 436 °C, an activation time of 20 min, and an impregnation ratio of 1.1 g ZnCl2/g BH, although the results after the single optimization of each response were quite different.
The timepoint of individual peak response after dobutamine administration varied between 5 and 15 minutes and analyses were performed from the maximal response values (Table 2).
Caspase-3 activation was determined by comparing maximal response values with untreated controls and results were plotted as increase over baseline.
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In addition, Figure 4a2 reveals a maximal response value which corresponds to 40°C temperature and 20 min heating time.
When the response to several ligand concentrations or with several protein concentrations was studied we used the maximal response value as θ.
A voltage pulse 0.2 ms in duration was gradually increased from threshold to maximal response value (the FBC model: 2 20 V, sham-operated mice: 5 25 V).
This requires (i) that the output adapts rapidly to changes in the input, and (ii) that the response does not saturate, i.e. max y output(t)) < max(O peak), which is the case if the proportionality coefficient α is low and/or the maximal response value max(O peak) is high.
This process yielded concentrations of EC50 and maximal response (efficacy) values for each concentration response curve (CRC).
Briefly, concentration response titration points for each compound were fitted to a four-parameter Hill equation (Hill 1910), yielding half maximal concentration (AC50) and maximal response (efficacy) values.
Concentration-response titration points for each compound were fitted to a four-parameter equation yielding concentrations of half-maximal activity (AC50) and maximal response (efficacy) values.
Concentration response titration points for each compound were fitted to the Hill equation (Hill 1910), yielding concentrations of half-maximal inhibition (IC50) and maximal response (efficacy) values.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com