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Data were normalized with respect to the maximal response obtained in the Pre-condition in each subject.
Under our assay conditions (cells were in serum-free culture medium for 24 h) both caspase-9 and caspase-8 displayed a larger basal activity than caspase-3 when compared with the corresponding maximal response obtained with STS.
Thus, the genes encoding chemokine (C motif) ligand 1, MMP13 and an IL2RB-like protein were elevated 42-, 54- and 11-fold respectively in TBL20 relative to the maximal response obtained for LPS stimulated BL20 cells.
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The maximal response was obtained at 6 8 mmol/L KCl (Fig. 3C) and represented 20% of the maximal contraction induced by 1 µmol/l PE.
A maximal response was obtained within 4 h.
A maximal response was obtained within 90 min and sustained over 120 min (upper part).
Sensitivity was calculated as pD2, where pD2 = −log[EC50], with EC50 being the concentration at which 50% of the maximal response was obtained (22).
> -wrap-foot> andiamcinolone and progesterone are slightly active in the yeast bioassay, but their maximal responses are only about 10% and 6%, respectively, of the maximal response as obtained with dexamethasone; thus, it is not appropriate to give an EC50 value or RCP.
Progesterone is also active in the GR-CALUX, but its response is only about 4% of the maximal response as obtained with dexamethasone bMifepristone is not active as an agonist in the GR-CALUX and acts as a pure antagonist in this assay Figure 3 shows the dose response curves for several natural and synthetic (gluco corticoids as obtained in de U2-OS cell-based GR-CALUX bioassay.
NC AgNPs were injected at different concentrations (0.07, 0.4, and 8.7 mg/L) and the maximal binding responses obtained on all four FCs were compared (Fig. 2).
In experiments, maximal response was often obtained only when the stimuli fell on disparate parts of the two retinas; these cortical cells were obviously disparity detectors, in contrast to others that gave maximal response when the stimuli fell on strictly symmetrically related parts of the two retinas i.e., on corresponding points.
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