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This process yielded concentrations of EC50 and maximal response (efficacy) values for each concentration response curve (CRC).
Briefly, concentration response titration points for each compound were fitted to a four-parameter Hill equation (Hill 1910), yielding half maximal concentration (AC50) and maximal response (efficacy) values.
Concentration-response titration points for each compound were fitted to a four-parameter equation yielding concentrations of half-maximal activity (AC50) and maximal response (efficacy) values.
Concentration response titration points for each compound were fitted to the Hill equation (Hill 1910), yielding concentrations of half-maximal inhibition (IC50) and maximal response (efficacy) values.
Concentration response titration points for each compound were fitted to a four-parameter Hill equation (Hill 1910), yielding concentrations of half-maximal activity (AC50) and maximal response (efficacy) values.
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In the melanization bioassay, the constrained analog exceeded the efficacy (maximal response) of the natural PBAN1-33 by a factor of 2 (at 1 nmol).
While the efficacy (percentage maximal response compared with reference compounds) was broadly similar between aleglitazar and other dual PPARα/γ agonists (Table 4), aleglitazar was the most potent ligand.
This allows for determination of relative efficacy, ε defined as the maximal response to test ligand divided by maximal response to a reference maximal activator (note than ε can exceed 1).
Relative efficacies of pheromones were normalized to the maximal response elicited by Z11 14 OAc.
Interestingly, LSP4-2022 robustly activates mGlu7 and its improved efficacy is apparent in vitro, where it induces a higher maximal response than glutamate in both calcium and GIRK assays (Supporting Information Figures S3 S5).
The concentration required for the half maximal response (EC50) value for AQP1 is expected to be higher than the KD value, since receptor occupancy is not identical to response efficacy [ 38].
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