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In fact, the consequences of the mutation at position 251 seem to be limited to a reduction of the maximal response at the highest diphenidol concentration, whereas the sensitivity of the construct ggTas2r1L251A appears largely unaffected.
This stimulation was both concentration- and time-dependent with maximal response at 0.5 mM BtcAMP and 4-h incubation, respectively.
GSK1059865 and JNJ10397049 displayed a non-surmountable antagonism of Orexin-A with depression of the agonist maximal response at OX1R.
In contrast, here we show in the heart that as little as 0.05 mM glucosamine altered substrate regulation in less than 1 hour with a maximal response at 0.1 mM glucosamine.
Furthermore, the effect of glucosamine on substrate oxidation was apparent at a glucosamine concentration as low as 0.05 mM with a maximal response at 0.1 mM, which was associated with significant increases in both UDP-GlcNAc and O-GlcNAc levels (Figure 1).
Unexpectedly, three subjects achieved a maximal response at day 14, in contrast to the expected 7 day peak time point, but in the absence of a day 14 sample in the BCG scar negative group, a formal comparison was not possible.
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Dose-dependent experiments showed an inverse-U shaped dose-response curve, with maximal responses at 0.25 mg/kg of LY37968, a plateau between 0.25 and 3 mg/kg, and loss of response at 4 mg/kg, i.p. (Fig. 1C).
Presumably as the result of a more elaborated electrode placement, we could also obtain our maximal responses at much lower stimulation intensities (about one order of magnitude), which means more specific effects in terms of the elements stimulated.
Dose-response curves show concentration-dependent effects for both cell types, with maximal responses at 25 50 μM.
Most of the genes affected with maximal responses at 2 ppm are thus associated with cell membrane and architecture, or otherwise, external aspects of the cell.
The authors showed in an elegant manner that the administration of BPA in a range from 5 to 50 000 µg/kg/d disrupts energy balance, with maximal responses at doses from 5 to 500 µg/kg/d.
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