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In sedated patients, starting intensity was set at 60 80 % of that resulting in maximal response and increased by 10%% every 3 min up to 100%%.
Full-field electroretinogram testing was normal for the right eye, with the exception of prolonged implicit time of the b-wave maximal response, and abnormal for the left eye, with reduced amplitudes of the isolated and maximal rod responses and a significantly prolonged 30 Hz cone flicker implicit time.
We then calculated the time of maximal response and the time needed for cells to return to basal levels (Table 1).
For cells that showed supersaturation (n = 13/81), we removed the data points that were below the maximal response for contrasts larger than the one evoking the maximal response and the fit was made on this reduced data set.
As shown in Figure 1A, exposure of UCB at a concentration of 1 µM for 24 h had no effect on the stimulus-response curve, maximal response, and fEPSP waveform.
The fitting of concentration response curves were calculated according to y = {ymax−ymin/1+(x/EC50)n}+ymin (where ymax = response in the absence of agonist, ymin = response remaining in presence of maximal agonist concentration, x = concentration, EC50 = concentration of agonist producing 50% of the maximal response and n = slope) with Kaleidagraph 3.5 software (Synergy Software, Reading, PA, USA).
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The films with the higher porosity would lead to the higher maximal responses and the larger corresponding film-thicknesses, or vice versa.
Metabolic and haemodynamic changes were quantified by maximal responses and by calculation of areas under the concentration time profile (AUC).
These kinetic curves were summarized with three numbers: maximum height (in mV), rising time (τrise) from stimulation onset to half-maximal response and falling time (τfall) from stimulation offset to half-maximal response.
From these curves, pD2 (the concentration required to produce a half-maximal response) and maximal response were derived and compared using an extra sum-of-squares F test. t-test was used where appropriate.
The second component of the model describes the continuous portion of the data with probability 1− πA as truncated log-normal distribution using a sigmoidal Emax function: where Base is the baseline value for free receptor, Emax is maximal drug effect, Cp is drug concentration, EC50 is the concentration of drug at half-maximal response, and γ is the Hill coefficient.
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