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The Mann-Whitney U test was used to compare the mean values of maximal release between GFP and Myc-CcaIncA 1 220 transfectants.
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However, the maximal release of PGE2 required a cross-talk between NK cells and MSCs involving IL-1β/IL-1R signalling.
NK-MSC cm was significantly superior to MSC cm in suppressing cytotoxic potential of NK cells indicating a need for a direct contact between the UC-MSCs and NK cells, for the maximal release of the cytotoxicity suppressant.
"The maximal release of hypocretin was not really related to arousal in the usual way.
It happened during waking for sure, but the maximal release was simply when people reported they were happy".
Percent specific lysis was calculated as ((experimental release – spontaneous release)/(maximal release – spontaneous release))*100.
Maximal release was obtained using 1% Triton-X 100.
Experiments in which spontaneous calcein release was more than 25% of maximal release were excluded.
The percentage of specific 51Cr release was calculated as (cpm experimental release - cpm spontaneous release)/(cpm maximal release - cpm spontaneous release) ×100, where cpm indicates counts per minute.
The mean of triplicate measurements was expressed as a percentage of specific lysis using the formula: (sample release – spontaneous release)/(maximal release – spontaneous release) ×100.
Allergen amounts necessary to trigger half maximal release of inflammatory mediator were determined.
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