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Single, double, or triplet spikes could easily be separated in the [Ca2+] transients by the maximal relative fluorescence change (Figure 2B).
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Figure 6 shows average fluorescence intensities and their maximal relative errors (A) and the cumulative distribution of maximal relative errors (B) For nearly 90% of the gene transcripts (n = 2,269), maximal relative errors of ORF-related signals were <60%.
However, the changes in the relative fluorescence intensities and shifts in the maximal emission wavelengths of the refolded mutants (370 nm for H956A and 358 nm for C997A) were less marked compared to the wild-type (Fig. 4A, C).
Fluorescent threshold was set to 50 relative fluorescence units.
The change in relative fluorescence over basal was calculated before normalization to the maximal response to ACh.
The relative fluorescence units (RFU) of eGFP increased gradually over time, reaching maximal fluorescence intensity after 50 min of incubation; eGFP expression level was also found to be dependent on RNA concentration.
relative fluorescence intensity.
RFU relative fluorescence unit.
Relative fluorescence unit.
Data represented as relative fluorescence unit (RFU) / μg protein.
c Relative fluorescence intensity of FCNPs in femoral tissues.
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