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This reaction time was established in preliminary experiments, which showed maximal reaction at 60 min. We used OASIS MAX (6 cc/150 mg; Waters Co). with N-vinylpyrrolidone/divinylbenzene hydrophilic lipophilic balanced copolymer mixed anion-exchange phase.
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Assuming a simple enzyme substrate reaction (Michaelis Menten equation) between thrombin and fibrinogen, the Michaelis constant (Km) value of fibrinogen at 2 g litre−1 (6 µM) represents half of the maximal reaction rate between thrombin and fibrinogen.
Here, the maximal reaction velocity has the same units as reaction flux.
The half-maximal reaction rates are set by m= δ/ g and μ= d/ γ.
Each reaction follows a Hill equation input-output relation given by Eq. (4) with a half-maximal response at m and a Hill coefficient of k.
Briefly, ligation product was maximal under slightly alkaline conditions with high substrate concentrations in a reaction at 42°C for up to 4 hours with dilute sortase A. Experimentally, the optimal reaction time is between 1 and 4 hours (Fig. 4A), after which EDTA may be added to quench the reaction.
A score of 30 indicates that the patient gives a maximal (normal) reaction and 0 indicates a minimal reaction.
Its activity was maximal at the reaction regime of 37 °C and pH 7.0 and was much more sensitive to Cu2+ and Zn2+ than to Li+ and Mn2+.
The oxidation rates were measured by monitoring the absorbance of CrVI at 355 nm, on a Milton-Roy 601 spectrophotometer, where the absorption of the oxidation products is maximal at the reaction pH.
The reaction at home?
Critical reaction at the time was mixed.
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