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Soluble antigen (OVA) applied to microporated skin was quickly absorbed and induced proliferation of adoptively transferred OVA-specific DO11.10 cells in a pore depth-dependent manner, with a maximal proliferation induced by 4 6 pulses (Fig. 2A).
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Our results indicate that the CD19/CD81 complex interacts with CD38 but this interaction is not required to induce proliferation in mouse B lymphocytes, suggesting that other receptors may contribute to the proliferation induced by CD38 in B lymphocytes.
Again, mock-infected MDDC induced minimal levels of CD4+ T cell proliferation (median of 0.4% of proliferated cells), and SEB-treated MDDC induced maximal proliferation (median of 93.1%).
However, addition of EGF, which induced a near maximal proliferation rate, resulted in a significant increase in DDX21 protein expression that was not further affected by insulin.
Maximal proliferation of LNCaP-abl cells was achieved at 0.001 n M of the synthetic androgen methyltrienolone (R1881), whereas 0.01 n M of this compound induced the same effect in parental cells.
Cryopreservation did not alter the maximal proliferation capacity of ConA or αCD3/αCD28 stimulated PBMC, whereas it did delay the proliferation.
Pretreatment with fibroblast growth factor-2 (FGF-2) increased the IGF-I receptor (IGF-IR) expression, and both FGF-2 and IGF-I were required for maximal proliferation.
Controls were considered as cells with a maximal proliferation rate (100%).
Moreover, the maximal proliferation was higher with QDova than with ovalbumin alone.
This media (NPC Growth media) was used to enhance growth conditions for maximal proliferation of cells.
The results suggest that HMGA2 expression is necessary for maximal proliferation of WZA LacZ cells.
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