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({EC_{textit{50}}}) value represents the molar concentration (M = mol/L) of an agonist that produces half of the maximal possible effect of that agonist.
Data are reported as maximal possible effect (MPE), calculated as 100% × [(drug response time - basal response time)/ cutoff time - basal response time)].
In the tail immersion and the von Frey tests, maximal possible effect (MPE) was calculated to facilitate inter-group comparisons, using a pre-determined cut-off value as follows: [ post-treatment score – pre-treatment score)/(cut-off value – pre-treatment score)].
The antinociception response was presented as percent maximal possible effect (%MPE) as defined by percent maximal possible effect = 100% × (drug response time – basal response time)/ cut-off time – basal response time).
The results of the tail immersion and hot plate tests were given with percentage of the maximal possible effect (% MPE), which was calculated using the following formula.
Since baseline latencies vary between animals, we use the percentage of maximal possible effect (%MPE) as parameter of thermal threshold values.
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Patients were instructed to apply the maximal possible force.
Data obtained were converted to percentage maximal possible antinociceptive effect (%MPE) as follows: (response latency - baseline latency)/ cut-off latency - baseline latency) × 100.
Expression values from 1528 microarray probe-sets were correlated with the results of 1) voluntary morphine consumption in two-bottle choice paradigm (C57BL/6J>129P3/J>DBA/2J>SWR/J) and 2percentnt of maximal possible analgesic effects (% MPE) of 3.6 mg/kg morphine measured by the hot-plate assay (129P3/J>DBA/2J>SWR/J>C57BL/6J).
On the 15th day, thermal withdrawal was measured after s.c. morphine (20 mg/kg), but not melatonin, and morphine tolerance was measured and expressed by MPAE%percentnt of maximal possible anti-nociceptive effect) of morphine.
For example, if a patient received the following adverse effect evaluations: vomiting 0, diarrhea 1+, dyspepsia 1+, acid suppressive therapy 1+, the gastrointestinal adverse effect ratio would be 3/9 = 0.33 or 33% (9 is maximal possible score for gastrointestinal adverse effects).
More suggestions(15)
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