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With 1 × 1 × 2 mm for each pixel as a voxel, the entire or target liver volume was calculated, whereas the area of HCC was calculated by dividing the maximal pixel number by 2. The powdered CDDP, IA-call® (Nippon Kayaku Co., Ltd, Tokyo, Japan), was solubilized in saline at a concentration of 100 mL/70 mL just before use.
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Here, M and N are the maximal available pixel number in x and y direction (M = 648, N = 488).
Bins are numbered 1 to 15 at every 6.67%% cut-off level of the maximal pixel radioactivity from 0%% (no activity) to 100%% (maximum).
Background-corrected maximal pixel intensity (PImaxcorr) in the left ventricle was determined with videodensitometry.
The maximal pixel value in the raw projection data is five.
Maximal pixel amplitude within the tidal image was divided by 10, which results in 10 different amplitude categories according to which each pixel was then assigned.
When the dilation was applied, the maximal pixel value overlapped by the structural element was found to replace the pixel value at the center of the structural element.
As with the basolateral membrane quantification, nuclear levels were calculated relative to a maximal pixel intensity of 255, after taking into consideration "background" pixel intensity.
Throughout the entire work, we used 4 as the pixel number threshold and 32 as the pixel intensity threshold.
The decrease in ratio of maximal pixel to nuclear area was even more sensitive senescence biomarker than the change in maximal pixel or nuclear area, each alone.
The intensities of maximal pixel and integrated fluorescence were measured and recorded for each cell.
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