Sentence examples for maximal pixel fluorescence from inspiring English sources

The morphometric analysis of the nucleus stained with the DNA fluorochrome DAPI shows an increase in nuclear area concomitant with the decrease in intensity of DAPI maximal pixel fluorescence [ 39].

Consistent with the data on the induction of senescence of A549 cells by the DNA-damaging drug Mxt (Fig. 3), dramatic reduction in the intensity of the maximal pixel fluorescence concurrent with the induction of p21WAF1 and p27KIP1 have been seen in cells treated with 0.5 and 1.0 μM of TSA.

As is show in Figure 6, treatment of A549 cells with Mxt in the presence of 2dG diminished both the degree of reduction of intensity of maximal pixel fluorescence of DAPI (DNA) and the decrease of mp/area ratio compared with cell growth in the presence of Mxt alone.

Briefly, the slides were scanned using the argon laser 488-nm line, and the cells separated on the basis of the DNA fluorescence area, maximal pixel intensity, integrated fluorescence and perimeter into cell clumps, doublets and single cells.

The intensities of maximal pixel and integrated fluorescence were measured and recorded for each cell.

Intensities of maximal pixel and integrated fluorescence were measured and recorded for each cell.

Thus, we studied the effect of exposure of A549 cells to one such inhibitor, TSA, by measuring the changes in intensity of maximal pixel DNA/DAPI fluorescence and nuclear area in the cells growing in its presence (Fig. 7).

As the ratio of intensity of maximal pixel of DAPI fluorescence to DAPI area (mp/area) was shown to be a sensitive biomarker of cell senescence, we tested the effects of higher concentrations of Mxt, shown before to lead to apoptosis rather than senescence on prolonged treatment in cultures (47).

Indeed, when the nuclear size represented by the area of the DNA-associated DAPI fluorescence was plotted versus the maximal pixel of DNA/DAPI fluorescence on bivariate distribution scatterplots (Fig. 3), it was apparent that senescent cells in the Mxt-treated cultures had increased area and distinctly decreased intensity of maximal pixel of DNA/DAPI fluorescence.

Thus, the presence on SAHF does not interfere with the use of the maximal pixel intensity of DAPI fluorescence as a marker of cell senescence.

The characteristic "flattening" of senescent cells was reflected by the decline in the density of staining (intensity of maximal pixel) of DNA-associated fluorescence [4,6-diamidino-2-phenylindole (DAPI)] paralleled by an increase in nuclear size (area).