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SUVmax was calculated as the maximal pixel activity within the region of interest.
Time-activity curves (TACs) were generated using the MIPAV software (Medical Image Processing, Analysis and Visualization; Center for Information Technology, National Institutes of Health, Bethesda, MD) with delineation of the kidney contour at 20% of maximal pixel activity within the organ using color threshold method.
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The maximum SUV (SUVmax) was recorded using the maximum pixel activity within the VOI.
Bins are numbered 1 to 15 at every 6.67%% cut-off level of the maximal pixel radioactivity from 0%% (no activity) to 100%% (maximum).
Background-corrected maximal pixel intensity (PImaxcorr) in the left ventricle was determined with videodensitometry.
The maximal pixel value in the raw projection data is five.
When the dilation was applied, the maximal pixel value overlapped by the structural element was found to replace the pixel value at the center of the structural element.
Maximal pixel amplitude within the tidal image was divided by 10, which results in 10 different amplitude categories according to which each pixel was then assigned.
This pixel activity depends on factors such as the pixel's luminosity or position on a screen.
As with the basolateral membrane quantification, nuclear levels were calculated relative to a maximal pixel intensity of 255, after taking into consideration "background" pixel intensity.
The decrease in ratio of maximal pixel to nuclear area was even more sensitive senescence biomarker than the change in maximal pixel or nuclear area, each alone.
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