To construct dynamic INa activation properties, phase plane plots (where current density or, equivalently, dV/dt is plotted against membrane potential [47]) are corrected for changes in driving force, current amplitudes normalized to the maximum peak current, and data fitted to a Boltzmann curve to determine membrane potential for half-maximal activation, V1/2, and the slope factor, k.
Currents were normalized to the maximum peak current for comparison.
The current reaches a maximal value (the peak current, <img src="http://journals.plos.org/plosone/article/asset?id=info?doi/10.1371/journal.pone.0018598.e010.PNG" class= inline-graphic"/> ) after <img src="http://journals.plos.org/plosone/article/asset?id=info:doi/10.1371/journal.pone.0018598.e011.PNG" class="inline-graphic"/> ms, corresponding to all channels being open.
The fabricated sensors could be used to efficiently detect pesticide residues, presenting good linear relationship between the trichlorfon concentration and the peak current, a maximal recovery of ca 98.0% and a low detection limit of about 4.0 × 10− 7 mol l− 1.
With 100 μM BzATP (maximal response) the time from 10%90%% peak current was 142.3 ± 10.2 ms and the rate of current decay from peak to 50% was 446.4 ± 38.9 ms (n = 7) (Fig. 3F).
In contrast, Nicholas et al., 2013 showed in hPSCs-derived GABAergic interneurons a steady increase of an unspecified K + current, reaching a maximal peak of ~1.5 nA only after 30 weeks of IVND, but Hartfield et al., 2014 showed no significant changes between relevant time-points in the average peak amplitude of K + currents in hPSCs-derived dopaminergic neurons.
The equation used for fitting the dose-response relationship was: Inorm= A/[1 + ([BmK I]/EC50 p ] + C, where Inorm is the measured and normalized peak current, E50 is the half maximal effective concentration, and p is the slop factor.
Peak current occurred at a ratio of ∼2 suggesting1, suggesthat that maximal CRAC channel activity requires binding of eight STIM1s to each channel.
Peak current values taken on the first application of a maximal concentration of ATP.
