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Both, cell growth and the fatty acid content, were very similar to the experiment with MgSO4, yielding a maximal optical density with 0.51 g l-1 and a maximum in fatty acid synthesis with 0.12 g l 1 magnesium, added as MgCl2.
OD600max: the maximal optical density measured at 600 nm.
For each sample, the optimal dilution (from 1 5 to 1 40) was established to obtain a maximal optical density (OD).
We observed large differences in growth rate, with the only strain isolated from Mus musculus (mm3up) growing significantly slower than the others and reaching a lower maximal optical density (Table 3).
Moreover, when overexpression of the vectors was induced by the addition of IPTG, only the vector expressing the CDS on its own caused a six-fold decrease in the maximal optical density of the culture whereas there was no effect for the vector with the ORF preceded by the 5′UTR.
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In standard LOD medium in the absence of furan aldehydes, JWCB001, JWCB018 and JWCB044, grew to similar maximal optical densities after 48 h at 75°C.
Further, all three strains reached similar maximal optical densities (~0.8 at OD680 nm) after 15 h incubation (Fig. 4a), suggesting that heterologous expression of these genes is not detrimental to C. bescii.
ELISA titers were expressed as those serum dilutions that lead to half-maximal optical density 450 (OD450) (titer50).
The aliquot was chosen for the maximal measured optical density to fall within the 0.45 0.6 A.U. range.
Amplitudes of photoconversion were estimated under actinic light as maximal changes in optical density at 550 nm comparing to the dark-adapted state.
AD0, blank anaerobic digestion assay; AD3, anaerobic digestion assay at 3 kg COD m-3 of CBW; AD6, anaerobic digestion assay at 6 kg COD m-3 of CBW; CBW, cork boiling wastewater, reactor influent; DPPH, 2,2-diphenyl-1-picrylhydrazyl; EC50, half maximal effective concentration; OD, optical density; VFA, volatile fatty acid.
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