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Amongst all the locally available inexpensive wastes studied, the strains exhibited maximal lipid yield coefficients (Y L/X) on 4 wastes viz., WCO, whey, WMO and fish waste (Table2).
Under these conditions, all the 5 strains showed an increase in biomass which varied from 3.77-7.42 3.77-7.42le the maximal lipid yield (Lmax, g L-1) varied from 0.84-1.48 g L-1 and whileachieved in 24–96 h afthe incubation.
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The maximum lipid yield, especially for oxygenated compounds was in the window of 150 250 °C.
However, many microalgae achieve maximal lipid yields only under stress conditions hindering growth and providing compositions not ideal for biofuel applications.
Only Idh activity in combination with the PPP allows for maximal lipid yields but it is not known whether the cytosolic Idh is subject to the same inhibition under nitrogen-limited conditions as its mitochondrial isozyme [ 35].
Continuous cultivation of C. curvatus was conducted under nitrogen-rich condition at a dilution rate of 0.04 h−1, the maximal lipid content and lipid yield were 56.7 % and 0.18 g/g, respectively.
The maximal specific lipid formation rate and lipid yield were higher under nitrogen-rich than nitrogen-limited conditions, which provided new opportunity for lipid overproduction from acetate-contained resources rich in nitrogen sources.
For shake-flask fermentation of T. fermentans without pH control, the maximal values of biomass, lipid content, and lipid yield were found to be obtained at initial pH of 6.5 [ 27], which can be explained from the variation of culture pH as indicated in Fig. 5a (at approximately 5.0 during most of the time).
At pH 4 the lipid yield and productivity reached maximum level (Fig. 5).
As shown in Fig. 5b, when cultures were maintained at pH 5.0, the values of biomass, lipid content, and lipid yield obtained in the presence of 30 mM [Ch][OAc] were the maximal, which were also very close to those achieved in the absence of [Ch][OAc].
Afterwards, net lipid productivity and lipid yield also declined.
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