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Histone acetylation rapidly increased after 3 h of treatment and reached maximal levels within 6 9 h.
As shown in Figure 5A, both Akt A-loop (T308) and HM (S473) phosphorylation reached maximal levels within 20 60 min of ghrelin addition (100 nM).
soxS mRNA levels surged to near maximal levels within the first few minutes of exposure to paraquat, a superoxide-producing compound, followed by a rise in mRNA levels of known SoxS-regulated genes.
Figure 3A shows that pPDK1 (S241) reached maximal levels within 5 10 min of ghrelin stimulation (100 nM), decreasing to ∼50% of the maximum by 60 min after stimulation.
However, analyses of BMP-stimulated hypertrophy suggested that ALP activity gradually increased over a 3 day period, while Col X mRNA reached maximal levels within 24 h.
There is a low level of proliferation in uninjured fins (0 hpa), but proliferation quickly increases to maximal levels within 48 hpa and then decreases.
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At the Baseline, only the mu (∼10-Hz) rhythms strongly rebounded after the visual stimuli given, usually starting at about 300 ms and reaching its maximal level within 700 ms after the stimulus.
In our experiment, thiamine treatment led to an induction of trans -and cis-piceide forms with maximal levels obtained within 4 and 2 dpt, respectively for each compound.
VDE transcript levels very rapidly increased after stimulation with blue and white light and close to maximal levels were reached within 1h (Fig. 7D).
The increase in ZEP2 levels is approximately 10-fold lower than ZEP1, but the kinetics of ZEP2 accumulation is faster and maximal levels were reached within 5 h of illumination (Fig. 7B).
Equally encouraging, conjugation to TPP+ has been used to deliver a wide range of lipophilic compounds to mitochondria in tissues and does so quickly, achieving maximal levels in the heart within 4 min of intravenous injection and maintaining these for >1 h [ 34].
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