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The HSV-1 VP5 and VP16 transcripts are expressed with leaky-late (γ1) kinetics and reach maximal levels after viral DNA replication.
In parental cells, Cdk1/cyclin B activity reached maximal levels after 12 13 h, and then declined because of cyclin B degradation (Fig. 5A, left).
A significant increase was then recorded after 1 h, 3 h and 6 h pt, reaching maximal levels after 3 h (3.8 times greater than the controls for both caspases).
While insulin-increased cell surface GLUT4 levels were relatively stable, FBS led to a somewhat transient increase in GLUT4 at the plasma membrane, reaching maximal levels after 7 10 minutes (Figure 4B).
In our animal model, serum TNF-α rapidly (within 1.5 hr) reached maximal levels after the Pb+LPS challenge.
Using a phospho-specific anti-pRB-antibody we detected - as expected - phosphorylation of pRB after stimulation with FCS which reached maximal levels after 14 h and persisted thereafter.
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This occurred in a time-dependent manner with the maximal level after 24 hrs of induction.
Our data also show that H2AX phosphorylation reaches 50% of its maximal level after 40 min. Therefore, even after extended exposure to etoposide at most 10% of the topoII-linked DSBs are converted to H2AX foci.
The inhibition reached its maximal level after 5 days, when approximately 80% of TA was abolished.
cPARP concentrations reached their maximal level after 20 min, after which they steadily decreased over 3 h.
Generation of this fragment reaches 50%% of its maximal level by 1 minute, and remains at elevated levels above 70%% of maximal level after 2 minutes.
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