Histone acetylation rapidly increased after 3 h of treatment and reached maximal levels within 6 9 h.
Figure 3A shows that pPDK1 (S241) reached maximal levels within 5 10 min of ghrelin stimulation (100 nM), decreasing to ∼50% of the maximum by 60 min after stimulation.
As shown in Figure 5A, both Akt A-loop (T308) and HM (S473) phosphorylation reached maximal levels within 20 60 min of ghrelin addition (100 nM).
soxS mRNA levels surged to near maximal levels within the first few minutes of exposure to paraquat, a superoxide-producing compound, followed by a rise in mRNA levels of known SoxS-regulated genes.
There is a low level of proliferation in uninjured fins (0 hpa), but proliferation quickly increases to maximal levels within 48 hpa and then decreases.
However, analyses of BMP-stimulated hypertrophy suggested that ALP activity gradually increased over a 3 day period, while Col X mRNA reached maximal levels within 24 h.
In most tissues, the autophagic activity reaches maximal levels within 24 hrs and then progressively decreases, whereas it further increases after 48 hrs in the heart and the soleus muscle [ 30].
In contrast, we observed rapid in vivo degranulation with maximal levels within 1 h after stimulation and with no significant increase in the extent of degranulation when the assay time was increased to 2 h.
In real-time cAMP measurements in both the presence and absence of extracellular Ca2+, cells stably-expressing AC2 showed a rapid increase in cAMP in response to CCh stimulation, and this increase reached maximal levels within 2 min.
It is apparent from Figure 4 that formation of alkaline labile phosphoprotein (i.e., aspartate phosphorylation) is faster than formation of alkali stable phosphoprotein (i.e., serine phosphorylation) and reaches near maximal levels within 1 or 2 s at 30 °C.
