Sentence examples for maximal laccase expression from inspiring English sources

Therefore, optimal concentration of copper ion for maximal laccase expression was determined ca. at 3 mM.

Maximal laccase production was determined using a second-order central-composite design and analyzed by the response-surface method.

The optimized conditions showed an enhanced laccase expression of 32.9% (from 538.8 to 803.3 U).

Methanol concentration, copper salts concentration and temperature were varied in order to enhance laccase expression in this heterologous system.

The maximal laccase volumetric activity (1.2 and 3.8 U/ml for non-induced and copper-induced cultures, respectively) was observed when the culturing was performed at a low agitation rate (0.9 kW/m3 s) and a high aeration flow rate (0.5 vvm).

Laccase expression was then induced by adding 0.04 mM isopropyl β-d-1-thiogalactopyranoside (IPTG).

In the present study, the maximal laccase production (1.27 × 105 U/g of dry substrate) (Fig. 3a) was obtained at pH 5.0.

To determine laccase expression levels, 9 putative genes encoding laccases with signal peptide were selected from the M. oryzae genome database for qRT-PCR.

Recently, miRNAs have also been reported to target laccases and function in the regulation of laccase expression at post-transcriptional level.

There are multiple cis-elements in promoter sequences of Arabidopsis laccases, indicating potential roles of transcription factors in regulating laccase expression [ 34].

At an optical density of 1-2 (ΔOD600), laccasexpressionon was induced by adding 0.1 mM IPTG.