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Spectra were acquired under automatic gain control (AGC) in one microscan for survey spectra and in three microscans for MS/MS spectra, with the maximal ion injection time of 100 ms per microscan.
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Automatic gain control (AGC) was set to 5 × 10 and maximum ion injection time (IT) was set to 200 ms.
Maximum ion injection time was 100 ms, and normalized collision energy was 35%.
Maximum ion injection times for MS1 and MS2 were 500 and 100 ms, respectively.
(A) Particle deposition with no ion injection.
We found that the maximal ion diffusion has a linear dependence on the number of sidechain monomers.
Ion injection and mass analysis parallelization can be employed up to resolution 130 000 (512 ms transient).
Code was written to enable ion injection, automatic gain control (AGC), manual and automated signal optimization, ion optic and mass analyzer calibration, quadrupole isolation, and MS/MS capabilities.
Prediction of ion injection time was enabled and the trap was set to gather 5 × 10 ions for up to 50 ms.
The ion drift is mostly controlled by E × B drift for both ion injections and flow-out.
An ion sponge of Geometry I with 2 trap layers (7 meshes) was used in a study with simultaneous ion injections at different locations.
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