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In both F9 and F9-Dab2 ceLRP6 LRP6 is rapidly internalized following Wnt3A treatment, with maximal internalization observed at 1 2 h, followed by its reappearance on the cell surface at 4 h.

As shown in Fig. 5C, for pro-B cells, CXCL12 treatment induced internalization of 35 40% of cell surface WT CXCR4 within 5 min, and maximal internalization of 50% was achieved at 60 min. On the contrary, CXCL12 binding to ΔT did not trigger endocytosis at all.

Detectable and maximal internalization of hypericin in RINm5F insulinoma cells occurred in 20 and 60 min respectively.

We now show that detectable and maximal internalization of hypericin in RINm5F insulinoma cells occurred in 20 and 60 min respectively.

As observed with Rog, maximal internalization of Rog-A in response to 1 μM spiradoline occurred after 20 min of drug treatment.

To address this question, whole blood was pre-incubated in the absence or presence of 10 μg/ml of 24F4A for 1 h at 37°C to obtain maximal internalization.

In addition, the maximal velocity of internalization at 37°C (Vmax⁡) was higher and showed two kinetic components in virulent amoebae (3.3 and 0.9  μmol/min) than in nonvirulent amoebae (0.4562  μmol/min), which only showed one component).

To quantify NK1-R internalization, we first standardized characterization of NK1-R expression and internalization at high magnification (63×).

TR-Tf internalization at each time point was quantified as previously described [53].

Then the samples were incubated at 37°C to initiate particle internalization at the indicated time points.

LPS-dependent activation of TLR4 led to partial receptor internalization at 5 min and a near complete internalization after 30 min for all monitored perturbations.

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