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If we denote the minimal and maximal intensity values (after applying the clipping function) as, respectively ymin and ymax, we have shown that the results hold (up to an arbitrary precision) in a "clipping-free" region y min ′ ≤ Δ t j E i α j ≤ y max ′, where typically y min ′ < y min + y max / 2 < y max ′ (with y min ′ and y max ′ as in Appendix 2).
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This filter assigns to every pixel the maximal intensity value of its 5×5 pixel neighbourhood.
The median of all pixels not within the object is taken (some basic outlier removal is performed) and subtracted from the objects maximal intensity value (this is designed to compensate for local background noise).
With a tenfold difference in signal, i.e. with one spot reaching intensity saturation in its centre peak pixel and the second spot with a maximal intensity value of 6,500, 0.16% of the lower signal was caused by overspill from the adjacent spot with the higher signal.
Also, to obtain maximal emission intensity values of polymers were investigated in different concentrations of DMF solutions.
In addition, in IMS of developing hippocampus formation (Figure 5), for each time point, intensity scale of C20-GD1 is normalized in order that the brightest pixels of C20-GD1 have 60% of the maximal C18-GD1 intensity value using the "Edit Mass Filter Parametrs" function of FlexImaging 2.0 software.
Images and numeric vibration intensity values during maximal inspiration were analyzed.
The fitting process was carried out for maximal intensity and for the area values of the selected peaks using Matlab cftool.
We determined the predominant BAG-1 immunostaining intensity and it is possible that the maximal intensity may provide improved prognostic value for metastatic potential.
To obtain maximal signal intensities without saturation effects, intensity values from Tiff images were integrated into one value per probe by the MAVI software (Version Pro 2.6.0, MWG Biotech, Ebersberg, Germany).
Fluorescence values of spots with maximal intensity (signal saturation) at the chosen laser intensity were extrapolated by linear regression, using values gathered from the two next lower laser intensities.
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