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This filter assigns to every pixel the maximal intensity value of its 5×5 pixel neighbourhood.
The median of all pixels not within the object is taken (some basic outlier removal is performed) and subtracted from the objects maximal intensity value (this is designed to compensate for local background noise).
With a tenfold difference in signal, i.e. with one spot reaching intensity saturation in its centre peak pixel and the second spot with a maximal intensity value of 6,500, 0.16% of the lower signal was caused by overspill from the adjacent spot with the higher signal.
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If we denote the minimal and maximal intensity values (after applying the clipping function) as, respectively ymin and ymax, we have shown that the results hold (up to an arbitrary precision) in a "clipping-free" region y min ′ ≤ Δ t j E i α j ≤ y max ′, where typically y min ′ < y min + y max / 2 < y max ′ (with y min ′ and y max ′ as in Appendix 2).
In addition, in IMS of developing hippocampus formation (Figure 5), for each time point, intensity scale of C20-GD1 is normalized in order that the brightest pixels of C20-GD1 have 60% of the maximal C18-GD1 intensity value using the "Edit Mass Filter Parametrs" function of FlexImaging 2.0 software.
Also, to obtain maximal emission intensity values of polymers were investigated in different concentrations of DMF solutions.
We determined the predominant BAG-1 immunostaining intensity and it is possible that the maximal intensity may provide improved prognostic value for metastatic potential.
The fitting process was carried out for maximal intensity and for the area values of the selected peaks using Matlab cftool.
Fluorescence values of spots with maximal intensity (signal saturation) at the chosen laser intensity were extrapolated by linear regression, using values gathered from the two next lower laser intensities.
The measured waveform showing a process of loading, unloading and oscillating is detected as a series of positive negative pulses, in which the peak value of the first negative pulse represents the maximal intensity of the stress wave.
The data of interest (averaged maximal intensity and peak area) for each ion were then normalized to the maximal value to avoid the digital arithmetic issues of overflow and underflow.
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