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Imaging of the freshly dissected brains of mice injected 24 h earlier with the loaded macrophages reveals that the green [metastasis] and red [microspheres] fluorescence overlap each other with maximal intensity in a posterior direction (Fig. 3).
Syndecan-1 showed maximal intensity in the cell nuclei, nucleoli and/or in the perinuclear area (Figure 1B, E and H, respectively).
In contrast, FGFR-1 (Figure 1D), known to form signaling complexes with FGF-2, was found only in the cytoplasm with maximal intensity in the perinuclear area, where it co-localized with syndecan-1 (Figure 1E, F).
The intensities observed at the other time points were then expressed relative to the maximal intensity in each time course.
According to theory, the X and Y coordinates of our source can be determined by identifying the pixel with maximal intensity in any of the four images.
DOI: http://dx.doi.org/10.7554/eLife.02040.013 10.7554/eLiFigure40.014 Figure 2 figure supplement 5. Cdr2p node number but not maximal intensity in each node increases with cell length.
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For this goal, it may be useful to analyze maximal intensities in scattering images as a function of deformation.
(C ) Scatter plot from EGFRGFP maximal intensities from filopodia from 6 neurons, showing a significant decrease in levels after treatment with Dyngo (EGFRGFP maximal intensities in DMSO: 1.229 ± 0.0074, n = 1403; EGFRGFP maximal intensities in Dyngo: 0.768 ± 0.005, n = 1401, p<0.001).
The maximal intensity may, in rare cases, induce transient muscular contractions.
Images were aligned by requiring maximal intensity overlap between the in-focus point-object image frame and the other frames in the scan.
(B ) Maximal intensity of EGFRGFP in filopodia within one neuron over time (2 min), before and after treatment (indicated by dotted line) with Dyngo.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com