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Instead, the inoculation of 100 ng Activin A led to a profound increase in the number of Langerin+ cells both in the epidermis and in the dermal layer, with a maximal induction observed 72 hrs after the injection.
In agreement with previous publications [13], [14], miR-210 was robustly induced in MCF7 and HCT116 by hypoxia (1% or 0.1% oxygen) at 24 and 48 hrs, with maximal induction observed at 48 hours in 0.1% oxygen (Figure 1A and Supporting Figure S1A).
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As shown in Figure 2A, increased GFPμ protein levels are first observed at 12 hours, and maximal induction is observed following 24 hours of exposure to 5 µM MG132.
Maximal changes were observed after six to nine hours.
The maximal induction ratio observed for split-CreERT2 was about 10 (Fig. 2B, 4B), which is in the same range as the reported in vitro induction ratios of CreERT and CreERT2 (about 7 to 15; [17], [17]).
Maximal induction was observed with 25 μ M of fenofibrate.
In addition, thiamine treatment induced pterostilbene synthesis with a maximal induction level observed also at 3 dpt.
The level of Brachyury protein began to increase 3d after surgery, and maximal induction was observed at 7d, as determined by Western blot analyses.
The levels of induction were different among the genes tested; the bMEC showed only a slight upregulation for TAP while the maximal induction was observed for BNDB4 (∼6-fold) and TNF- α (∼14-fold).
In addition, the induction of fibronectin, vimentin, and α-SMA expression by β2-M in vitro was dose dependent, and the maximal induction was observed following treatment with 25 μM of β2-M (data not shown).
Brachyury mRNA was induced after treatment of tubular epithelial cells with 1 ng/ml TGF-β1, and the maximal induction was observed at 5 ng/ml TGF-β1, as demonstrated by qRT-PCR.
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