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As shown in Figure 2A, increased GFPμ protein levels are first observed at 12 hours, and maximal induction is observed following 24 hours of exposure to 5 µM MG132.

Maximal induction is achieved at 6 hours with 300 μM DCA.

Maximal induction was observed with 25 μ M of fenofibrate.

Boterenbrood and Nieuwkoop (Boterenbrood and Nieuwkoop, 1973) have shown that maximal induction was achieved upon culture with ventral vegetal cells of early gastrulae.

The level of Brachyury protein began to increase 3d after surgery, and maximal induction was observed at 7d, as determined by Western blot analyses.

The levels of induction were different among the genes tested; the bMEC showed only a slight upregulation for TAP while the maximal induction was observed for BNDB4 (∼6-fold) and TNF- α (∼14-fold).

In addition, the induction of fibronectin, vimentin, and α-SMA expression by β2-M in vitro was dose dependent, and the maximal induction was observed following treatment with 25 μM of β2-M (data not shown).

The Hif1-α mRNA was expressed basally and this expression was significantly higher in stimulated than in unstimulated-cells 1 h after the beginning of the treatment, and maximal induction was achieved after a 2 h incubation period.

Brachyury mRNA was induced after treatment of tubular epithelial cells with 1 ng/ml TGF-β1, and the maximal induction was observed at 5 ng/ml TGF-β1, as demonstrated by qRT-PCR.

While significant increases in TNF-α and IL-6 expression were evident at 120-day-old PR-SET7 ΔHepA livers, maximal inductions were detected 4 months later in P240 livers (Supplementary Fig S3D and E).

Maximal GFP induction was defined by the response to 100 ng ml−1 IL-6 (half-maximal induction at ∼1 ng ml−1 IL-6).