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In contrast, hSLFN12 mRNA expression was up-regulated by LPS in HMDM from multiple independent donors, with maximal induction at the latest time point examined (∼4 5 fold, Figure 3C).

For permethrin, both Camk1g and Gpd1 had maximal induction at 6 h preceded by slight elevations at 3 h.

EGC also time and dose dependently increased GCLM mRNA expression in THP-1 cells with maximal induction at 4 h.

CD59 upregulation by atorvastatin in hypoxia was dependent upon increased steady-state mRNA, with maximal induction at 8 hours returning to baseline 16 hours post-treatment.

Gene expression of MMP-3 and MMP-13 was detected at 6 hours with maximal induction at 48 hours in unstrained constructs treated with telopeptides or the NH2-FN-f.

Induction of reporter activity in AI cells occurred at concentration of 0.05  μ M, and achieved maximal induction at concentration of 0.10  μ M, almost a nine-fold increase (P<0.01).

Maximal GFP induction was defined by the response to 100 ng ml−1 IL-6 (half-maximal induction at ∼1 ng ml−1 IL-6).

When assessing genistein's activity in BG1Luc4E2 cells, we found genistein was able to superinduce luciferase (190 ± 9% of maximum E2 activity) and was, not surprisingly, more potent than either rhodoeosein or emodin with half-maximal induction at 0.73 μM ± 0.15 μM (approximately 4 orders of magnitude weaker than the E2 standard).

The fixed elimination rate model was optimized at exposures near maximal induction; thus, at high exposures, the KBILE_LI used in the fixed model is not very different from the KBILE_LI derived in the inducible elimination model.

In agreement with previous publications [13], [14], miR-210 was robustly induced in MCF7 and HCT116 by hypoxia (1% or 0.1% oxygen) at 24 and 48 hrs, with maximal induction observed at 48 hours in 0.1% oxygen (Figure 1A and Supporting Figure S1A).

The induction appears to be biphasic, with the maximal induction accomplished at 0.1 μg/mL (Fig. 5A).