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Exogenous SMase led to a dose-dependent increase of MMP-1 with a 3.1-fold increase at concentrations as low as 0.005 U/ml and maximal increase at a concentration of 0.125 U/ml (Figure 4).

Expression of the CXCL8_L2 gene was quickly and significantly upregulated in peritoneal leukocytes at 6 48 h after zymosan injection, with a maximal increase at 6 h of inflammation (Fig. 6a).

The half-maximal stimulation occurred at 19 ng/ml, and the maximal increase at doses of ≥ 50 ng/ml (39).

However, the response was less than that of ET-1 (∼ 3.6-fold) and was delayed (maximal increase at 1 h) (Fig. 1A).

Treatment with TCDD showed a nonmonotonic increase in Epcam mRNA with a maximal increase at 0.1 nM followed by a decrease at higher concentrations.

A time-course study revealed that exposure of MEF cells to MSU crystals (2 mg/ml) enhanced IL-1β secretion, with a maximal increase at 36 h, while that of TGF-β1 had already occurred by 12 h.

WCh and IPL produced a time-dependent increase in NE outflow in the mpFC reaching a maximal increase of 60% at 120 min and 65% at 60 min, respectively.

In stressed groups, MCh and IL produced a time-dependent increase of norepinephrine outflow, which reached a maximal increase of 70% at 120 min and 60% at 60, respectively.

A significant increase was observed at a PDGF concentration as low as 5 ng/mL, whereas the maximal increase was obtained at a concentration of 20 ng/mL.

We observed that PlGF treatment of HMEC resulted in a time-dependent increase in SKA2 mRNA expression with maximal increase of ∼10-fold at 4 h.

Figures 3 and 4 show the distribution of the changes in arm volume and the maximal increase in arm circumference at two adjacent measurement points 12 months after surgery.