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By using two-photon excitation microscopy, this maximal imaging depth can be increased to up to 1 mm (Denk et al. 1990).
The measured scan depth was 12.55 mm; the axial resolution of the system near the zero-delay line was approximately 7.0 μm in air; and the sensitivity was 99 dB near the zero delay line, with a 51 dB drop at the maximal imaging depth.
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The maximum imaging depth, however, is limited in most tissues to ~1 mm [ 8, 9].
In addition, the calibration mirror image limits imaging depth [ 19].
We observe somewhat better imaging depth and clearer images in NP samples.
The spectrograph probing window ΔℓS depends on the maximal measurable path length difference by the spectrograph ΔOPLmax (or imaging depth dmax = ½ΔOPLmax) and the sensitivity roll-off with ΔOPL of the spectrograph.
The OCT images typically have deep (2-3 mm) imaging depth with 5-10 μm transverse resolution.
Thus, the method proposed does not affect the imaging depth.
An imaging depth improvement factor of 1.5 is demonstrated.
The probe showed high ratiometric imaging resolution and deep-tissue imaging depth of over 180 μm.
Systems typically reach an imaging depth of 2-3mm.
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