Sentence examples for maximal flux rate from inspiring English sources

For each of these metabolites we estimated the maximal flux rate through its exchange reaction under at least 90% maximal growth rate, and compared it to that measured experimentally across the 59 cell-lines for which data exist.

In this case, in silico IC50 values are calculated by estimating the maximal flux rate carried by the target reaction when growth rate is set to 50% of its maximal value.

Here we enforced a positive flux through the target reactions that is 50% of the maximal flux rate it is able to carry (our results are robust to various activation thresholds; Supplementary file 1D).

Utilizing the RPMI-8226 model we first sampled the solution space and obtained 1000 wild-type flux distributions under maximal growth rate, in which the MLYCD reaction is forced to be active in a rate that is at least 50% of the maximal flux rate it can carry.

We therefore revised our model to accommodate two SERCA subtypes and manipulated the maximal flux rates through the influx and clearance mechanisms to best simulate the peaks of transient [Ca2+]i and the time constants following depolarization in normal cells under different conditions (Fig. 3A).

Maximal oxygen flux rates (state uncoupled, state U) were obtained by titration of the chemical uncoupler carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP).

Then, for all reactions in the model, we computed the minimal and maximal flux at this growth rate.

Next we tested if individual simulations show overflow-like metabolism or not. Figure 4 shows a frequency diagram of the reduction of the flux through the high-yield pathway at maximal growth rate, as a percentage of the maximal flux through the high-yield pathway.

The metabolic flux distribution and the changes of metabolic fluxes under several perturbed conditions were examined by flux variability analysis (FVA) [ 26, 27] that calculates minimal and maximal flux values of each reaction for an objective function of maximum cell growth rate.

Flux rate increased with increasing pressure.

Second, reactions with low flux variability were those whose absolute values on flux variability were less or equal to 50% of the maximal flux variation along the entire distribution.