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The maximal flux position along MLT is moving from pre-midnight sector in quiet time to post-midnight one before and during SSC and moving back during recovery phase.
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Second, reactions with low flux variability were those whose absolute values on flux variability were less or equal to 50% of the maximal flux variation along the entire distribution.
We use the maximal flux value as the corresponding reaction flux capacity.
For each reaction in the network, the reaction flux capacity is the set to the maximal flux value of the reaction from the flux distributions.
Under physiological conditions, each reaction will have a limited maximal flux due to thermodynamic constraints.
'0' means the knocked-out enzyme resulting no flux of biomass or CO2 fixation, while '1' means there is no influence on maximal flux of biomass or CO2 fixation.
In any optimal FBA solution with a maximal flux of one through R01, a net flux of one is required from R01 to R05.
When performing Flux Variability Analysis [ 17, 50], we selected reactions that could increase or decrease by 25% of their maximal flux value for further analysis.
Simulations of CYP8B1 knock-out with the HepatoNet1 model using conventional CBM alone changes the maximal flux towards cholate from 0.898 to 0.1, without impacting on the maximal flux towards chenodiol.
Then, for all reactions in the model, we computed the minimal and maximal flux at this growth rate.
We concluded that more reactions have no influence on the maximal flux of CO2 fixation than biomass.
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