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From these, we randomly selected 25 cells for each construct and assembled fluorescent onset curves from the time of transfection to the point when maximal fluorescence was reached.

Finally, fluorescence was measured after 30 min of incubation at 37°C using a microplate Fluoroscan Ascent spectrofluorometer (excitation at 540 nm and emission at 590 nm), as preliminary experiments have shown that maximal fluorescence was obtained at that time independently of the dose of squalamine.

Furthermore, T selectivity (T/NS) after topical ALA at the interval of maximal fluorescence was higher than at the interval directly after application.

With fluorescence cryomicroscopy localisation of fluorescence in the skin at the interval of maximal fluorescence was determined after both administration routes.

Fluorescence and pressure were monitored continuously, and the point of maximal fluorescence was taken as the point were pressure stabilized at a steady state.

Subsequently photodynamic therapy at times corresponding to maximal fluorescence was performed using two light doses, 100 and 250 J cm-2.

Accordingly, the variations in maximal fluorescence are consistent with the idea that helix D coiled-coil formation helps stabilize the AB/CaM complex.

The maximal SG fluorescence was obtained by full permeabilization of the cells using 0.05% Triton X-100.

Similar to the wt DBD, many of the combinations facilitated AND transcriptional logic at PTAN, and maximal GFP fluorescence was observed only in the presence of both inducers.

The maximal increase in fluorescence was reached in less than 2 min.

Finally, the maximal increase in fluorescence was reduced in the complementation assay (AB vs AB#, ABCD vs ABCD#, ABCD-L609R vs ABCD -L609R; Fig. 5ABCD -L609Ras partially related to the kinetics oFighe increase in D-CaM fluorescence (Fig. 5A).