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The maximal fluorescence level in the dark-adapted state (Fm) and the maximal fluorescence level during illumination (Fm') were measured by a 0.8s saturating pulse at 5000 µmol m−2 s−1.
NPQ was calculated as (Fm/Fm' -1 where Fm is the maximal fluorescence level in the dark [ 47].
The maximal fluorescence level (FM) in the dark-adapted state was measured by a 0.8-s saturating pulse at 8000 μmol m−2 s−1.
The maximal fluorescence level in the dark-adapted state (Fm), the fluorescence emission from leaf adapted to actinic light (F') and the maximal fluorescence level during illumination (F'm) were measured by a 0.8 s saturating pulse at 5000 μmol m-2 s-1.
The maximal quantum yield of PSII photochemistry was measured in dark-adapted leaves from the maximal fluorescence level (Fm) and the initial level (Fo): (Fm-Fo)/Fm = Fv/Fm.
The maximal fluorescence level in the dark-adapted state (Fm) were measured by a 0.8 s saturating pulse at 8000 μmol m-2 s-1 after 20 min of dark adaptation.
Similar(52)
Camera gain was adjusted to give baseline maximal fluorescence levels of 40 100 (arbitrary units) of a maximal 8-bit signal output of 256.
(D ) SNR curves for CaRuby-Me at different maximum fluorescence levels (maximal photon numbers indicated next to the corresponding curves).
To keep filters moist they were transferred on a wet paper towel and cells were dark adapted for 20 min before maximal chlorophyll fluorescence level F m was determined.
The steady-state value of fluorescence (Fs) was thereafter recorded and the second saturating pulse at 8000 μmol m-2s-1 was imposed to determine the maximal light-adapted (F'm) fluorescence level.
The analysis of the bacterial samples further confirmed that the transformant bacteria exhibited strong levels of brightness (a maximal fluorescence emission at 495 nm upon excitation with the blue light) similar to the levels previously reported for the FbFP expression in E. coli and C. albicans [7].
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