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The MDA Cp values indicated time (hours) required to reach half of the maximal fluorescence in each well.
In addition, we find that maximal fluorescence in the cytosol of mammalian cells requires the iron-sulfur cluster assembly proteins ISCU and NFS1.
Results: After exposure to 5-aminolevulinic acid, cervical tissue showed maximal fluorescence in dysplastic cells relative to normal cells, with negligible stromal fluorescence.
In controls, maximal fluorescence in the anterior chamber was 3.1 +/- 0.8% of blood fluorescence.
The steady-state value of fluorescence (Fs) was thereafter recorded and a second saturating pulse at 8000 μmol·m-2·s-1 μmol·m-2·s-1 μmol·m-2·s-1the maximal fluorescence in the light-adapted state (Fm').
Antibodies were titrated for maximal fluorescence in sections of inflamed tonsil [42] and validation of the staining method was done using isotype antibodies and PBS as negative controls.
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In all cases, the concentration of BV required for half-maximal fluorescence in whole cells (2.3 15.5 μM) was lower than the concentration (80 μM) used for screening the libraries.
However, the average normalized fluorescence intensity for bins anterior to the position of maximal fluorescence intensity in the nucleus in MiTMAB-treated cells was significantly lower than that of control cells, suggesting a rearward redistribution rather than general alteration in adhesion protein levels.
Quantification revealed a significant increase in the average normalized fluorescence intensity for all bins posterior to the position of maximal fluorescence intensity in the nucleus in MiTMAB-treated cells (Figure 5I), and the rearward redistribution of adhesion proteins occurred whether or not the neuron displayed a trailing process.
The maximal fluorescence level in the dark-adapted state (Fm) and the maximal fluorescence level during illumination (Fm') were measured by a 0.8s saturating pulse at 5000 µmol m−2 s−1.
DENV-2 has been reported to reach maximal fluorescence staining in the midgut 7 days after infection of a Chetumal strain [23] whereas no data have been published for CHIKV- infected mosquitoes.
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