For example fluid absorption capacity reflects the larger urinary volumes in adults, the outer cover materials are selected to avoid rustling noises and the anatomical shapes are designed for maximal fit and wearing comfort [ 8, 9].
The line graph normalised the responses (to maximal) and fitted a sigmoidal function.
Light saturation is defined at 90%% of the maximal ETR fitted by the model and was derived by solving equation (1).
The concentration effect profile is depicted in Figure 2 with maximal likelihood fitted parameters from equation 2. Reduction in bacterial counts with vancomycin occurred in a concentration-dependent manner, as seen with the steep sigmoidicity constant (H) and low EC50 against most isolates.
The dissociation constant (Kd) value was calculated as the free concentration of radioligand at half-maximal binding by fitting data to the Hill equation and by linear Scatchard transformation.
where affinity can be any binding measurement, in our case the docking score; x is derived by fitting the maximal ligand efficiency (LE max ) values from all 12 docking screens against HAC, to a logarithmic function of the form: ln ( L E m a x ) = k - x ln ( H A C ) (2).
In order to apply a SILE metric for our data, a value for x, for Equation (1), was obtained by fitting the maximal LE (LE max ) values taken from Figure 6 to the function in Equation (2) (see Additional file 1).
Thereafter, a virtual sphere was used to describe the femoral head, with the diameter fitting this maximal diameter.
For quantification of the results, the ED50 values (effective dilution leading to 50% loss of activity) were deduced from the sigmoid ELISA curves [log intestinal fluid dilution) vs. response (% maximal binding)] by fitting them using a standard slope model of software Prism (GraphPad).
By comparison, the variant proteins that exhibited aberrant or weak heme binding by visible spectroscopy required much greater (at least 5-fold higher) heme concentrations for maximal quenching, and curve fitting suggested dissociation constants at least 5-fold higher (data not shown).
