For each time point, a color is defined in the proteome map, which is then assigned to spots that have their maximal expression level at that time point (Fig. 17b).
β a is the maximal expression level of promoter.
β r is the maximal expression level of promoter.
As a complementary analysis, we classified 1632 ring-specific, 1378 trophozoite-specific, and 1274 schizont-specific genes according to their maximal expression time-point.
The animals were killed 2 h after formalin injection for the analysis of c-fos activation, since this is considered the time point of maximal expression of the proto-oncogene (Coggeshall, 2005).
To examine this, qRT-PCR was performed on B. pahangi mosquito-derived L3 and on day 10 p.i. L4, the time point at which maximal expression of bpa-miR-5364 bpa-miR-5364 bpa-miR-5364hangi β-tubulin as a normalising gene [ 45- 47].
In that respect, our results uncover a striking difference between lipopolysaccharide and viral treatments concerning head kidney transcriptomic dynamics: in attenuated and active IHNV groups, the complement related serine proteases and anaphylatoxin receptors (C3aR) maintain up regulated expression levels at both time points, with the maximal expression (>5 6 fold change) in active viral groups.
Because input noise falls to zero at maximal expression, the sole remaining noise at that point is the output noise, and this uniquely determines the parameter α.
To compare developmental gene expression profiles, published time series of high-throughput RNA expression data for DD and DP8 were expressed as percentage of maximal expression and stage-matched to new RNA expression data for DL, PP and DF.
Clusters containing overrepresented E2F motifs in the upstream regions of their genes are observed to have maximal expression at any of the seven post-infection time points.
