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In our buffer system, the maximal emission for both NATA and MSI1 is 350 nm.
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Laser light at the maximal emission of the acceptor (514nm for YFP) is used to destroy YFP emission within a desired location within the cell.
The maximal emission wavelength for Trp136 is blue shifted about 10 nm compared to Trp162, consistent with a less polar environment for Trp136.
However, the changes in the relative fluorescence intensities and shifts in the maximal emission wavelengths of the refolded mutants (370 nm for H956A and 358 nm for C997A) were less marked compared to the wild-type (Fig. 4A, C).
equipped with filter wheel sets for maximal excitation at 485 nm and maximal emission at 516 nm.
The cell lysates were diluted in cell-impermeant 10 µM FluoZinTM-3, tetrapotassium salt (Invitrogen) in 10 mM Tris-HCl (pH 7.4), and incubated for 30 sec prior to measuring the maximal emission at 516 nm (excitation at 494 nm) using a Varioskan (Thermo Electron Corporation).
To determine the optimal pH value for MDA derivatives, fluorescence intensity of mBSA was analyzed, and a maximal emission intensity detected at around pH 7.0 (Fig. S6A), making it suitable as a fluorescent probe in vitro and in vivo.
The ANS binding data for this protein, depicted in Figure 4e, are also nonlinear, with maximal emission at 510 nm.
Also, the optimization of the concentrations to obtain maximal emission intensity is investigated in DMF.
Fluorescence titration of HFlu suggests that the fluorescent intensities at the maximal emission decrease upon the addition of Co acac 2.
Also, to obtain maximal emission intensity values of polymers were investigated in different concentrations of DMF solutions.
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