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equipped with filter wheel sets for maximal excitation at 485 nm and maximal emission at 516 nm.
The ANS binding data for this protein, depicted in Figure 4e, are also nonlinear, with maximal emission at 510 nm.
Both IAs emitted an intense fluorescence when excited at 444 nm with maximal emission at 553 554 nm.
A Vilbert-Lourmat VL-215M lamp was used as UV-B light source, with maximal emission at 312 nm, in combination with an 0.1 mm cellulose acetate filter (Clarfoil, Courtaluds Chemicals, UK) in order to screen out any UV-C contribution.
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To determine the optimal pH value for MDA derivatives, fluorescence intensity of mBSA was analyzed, and a maximal emission intensity detected at around pH 7.0 (Fig. S6A), making it suitable as a fluorescent probe in vitro and in vivo.
A maximal emission was obtained at 337 nm.
The Ngb protein has 3 Trp and 4 Tyr, and the maximal emission wavelength is at 338 nm, which indicates that there is only a small exposure to the protein surface among these Trp residues [ 14].
To induce such resonance, silver or gold particles were excited with visible-wavelength laser lines (561 or 640 nm), and the maximal emission signal was collected at a shorter wavelength (i.e., higher energy state).
Fluorescence titration of HFlu suggests that the fluorescent intensities at the maximal emission decrease upon the addition of Co acac 2.
Laser light at the maximal emission of the acceptor (514nm for YFP) is used to destroy YFP emission within a desired location within the cell.
3A, 3C and 5C). Figure 5C also shows that Microcoleus exhibited the classical TL oscillation pattern (maximal TL at the 2nd and 6th flashes) but their intensity and maximal emission pattern declined after exposure to excess light.
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