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The effects of estradiol on human GnRH upstream promoter activity in JEG-3 cells depends on the amounts of estrogen receptor (ER) alpha expression vector co-transfected, with the maximal effect obtained at the amount of 1.0 μg of ER expression vector cotransfected.
In vitro, Sb supernatant markedly enhanced wound repair (Figure 1A) in a dose-dependent manner (not shown) with a maximal effect obtained with a 1/8 dilution.
A pretreatment of hippocampal neuronal cells with resveratrol (15 40 μM) significantly reduced A β25 35-induced cell death in a dose-dependent manner, with a maximal effect obtained at 25 μM.
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In order to analyze the proliferative effects induced by CXCR4 activation, the obtained mammary carcinoma primary cultures were starved for 24 h and treated with 25 nM SDF-1 for further 24 h (the concentration was chosen as the maximal growth stimulatory effect obtained in previous experiments on human breast cancer cell lines [ 19]), and then MTT cell viability assays were performed.
The maximal effect was obtained at a 25-μM concentration which is similar to concentrations of PPF used clinically.
The results showed that triterpenes-induced JNK activation was abrogated by catalase in a dose-dependent manner and the maximal effect was obtained at 500 U/ml.
The concentration at which NK-4 exerted its maximum effect differed when assessed by growth or neurite-outgrowth: a maximal effect was obtained at 250 nM as assessed by cell growth, whereas a higher dose of up to 2.5 µM was needed for neurite-outgrowth.
The maximal effect was obtained after 72 hours incubation with SI 34 10 μM.
The maximal effect was obtained at 15 min for p44/42 kinase and at 45 min for SAP/JNK.
In 90% of adult cases, maximal effect is obtained with inspired concentrations between 3 and 5 ppm.
The maximal effect was obtained using a final concentration of 10-20 nM (Fig. 6E), in agreement with values reported in the literature (Cheung and Wong, 2008).
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