In contrast, when δ subunits were co-expressed with α and β, flupirtine acted as a positive allosteric modulator and markedly enhanced maximal current amplitudes.
Approximate analytical expressions for all characteristics of the system (concentration profiles, maximal current, etc).
Source data for time to 50% maximal current can be found in Supplementary Table S2. Figure 3: Voltage Dependence of NaV1.1 Conductance and Fast Inactivation.
Time to 50% maximal current is plotted versus voltage for WT and A1273V NaV1.1 channels at 37 °C (c) and 40 °C (d).
The theory also predicts a complicated dependence of the maximal current on the bulk solution concentrations of bromate and protons.
The same pattern was present in recordings from GFP-D2 slices, however the maximal current amplitude produced in cells from GFP-D2 slices was significantly smaller than seen in WT (Fig. 2F).
To determine the wavelength sensitivity of this current, 3T3-L1 differentiadipocytescytes were exposed to a 400 600 nm spectral ramp and the maximal current was elicited at ~450 480 nm blue light (Fig. 1C).
The maximal current becomes much higher, exceeding the one limited by the bromine diffusion from the bulk solution, and it depends essentially on the kinetic layer thickness.
The maximal current becomes much higher than the mass-transport of halogen from the bulk can sustain, depending essentially on the kinetic layer thickness.
The maximal current density of 220 mA cm−2 and power density of 65 mW cm−2 were obtained at ambient conditions.
The theory predicts a complicated behavior of the maximal current as a function of the diffusion layer thickness (or of the disk rotation rate for the RDE technique), with a maximum and a minimum separated by the range with an anomalous variation: increase of the maximal current with increase of the diffusion layer thickness ("autocatalytic interval").
