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However, maximal contraction to ACh in 2ws (168 ± 8.4 %KCl) was greater than for 10ws (142 ± 2.3 %KCl; P < 0.02).
In these same tissues, maximal contraction to PE (10 µM) was reduced by ∼80%.
A recovery of 1 minute was allowed between each maximal contraction, to minimize fatigue.
For vasodilation studies, rings were pre-constricted to 75% of the maximal contraction to phenylephrine prior to addition of acetycholine or sodium nitroprusside.
Contraction amplitudes (CA): Maximal CA is difference between baseline pupil size (BS) and the normalized pupil size (NPS) at maximal contraction to light.
Relaxation to the vasodilator agents is expressed as percentage reduction of the maximal contraction to PE. Mean intergroup differences were tested by repeated measures analysis of variance (ANOVA), followed by Scheffé's least-significant-difference test.
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Maximal contractions to 60 mmol/L KCl were reduced in grafts from L -arginine fed dogs.
In proximal coronary artery segments, maximal contractions to sumatriptan (16+/-18 16+/-18traction to 100 mM KCl) and DHE (5+/-4%) were nof significontractionerento
Three submaximal contractions will be allowed prior to three maximal contractions to allow for familiarization with the procedure.
Students are asked to perform one isometric sub-maximal contraction, to ensure that the correct action is performed.
Secondary Endpoints Each active-treatment group showed significant improvements in line severity at maximal contraction relative to baseline ratings (p < 0.001), in contrast to the placebo group, which did not improve.
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