In contrast, the short coiled-coil construct, NEMOwCcc8aa, behaves like NEMOw, and the binding curve does not reach a plateau at the maximal protein concentration tested, 52 μM.
Although Km values were higher than the maximal sugar concentration tested, the transport capacity for D-xylose at this concentration was estimated for comparative purposes: 33 ± 6 mmol.h-1.
Both assays showed that resveratrol (1 30 µM) strongly attenuated SNP-induced toxicity, producing a significant effect at 10 µM and a maximal one at the highest concentration tested (Fig. 5A, B).
Furthermore, the concentration dependence of CtpSE277R k cat was less steep than wild type, with CtpSE277R retaining 48% of its maximal activity at the highest enzyme concentration tested (8 μM).
In mixed (glial/neuronal) hippocampal cultured cells, treatment with the NO donor SNP (100 μM) resulted in cell damage and resveratrol (5 ± 25 μM) treatment increases cell survival, and this protective effect was significant at 5 μM and maximal at the highest (25 μM) concentration tested.
In heterozygotes, the fraction of unblistered wings is already maximal at 12 µM TSA, the lowest concentration tested, and decreases slightly at higher TSA concentrations, presumably because a minimum level of Rpd3 activity is required for normal development (Fig. 5B).
