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For both methods, calcium measurements were calibrated to the maximal calcium load produced by ionomycin (5 μM, 100% response).
Bradykinin typically produced a biphasic response; at 30 μM concentration, [Ca2+]i raised abruptly to a peak that attained 44±2%2% of the maximal calcium load produced by ionomycin (5 μM, 100% response), then declined to a plateau of elevated [Ca2+]i which remained fairly constant until drug washout.
Exogenous application of ATP and ADP (1 mM) transiently increased intracellular [Ca2+]i reaching, respectively, 43 ± 4% (n = 8) and 25 ± 8% (n = 3) of the maximal calcium load caused by ionomycin (5 μM, 100% response) within 20 sec from drug application.
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Twitch tension under maximal calcium amounts to 1 2 mN/mm2.
During the synchronization events the maximal calcium levels were typically much higher than during the independent oscillations.
Moreover, an inverse correlation between arterial calcium load and arterial wall metabolism was found.
Adding to our calcium load are many foods that are fortified with calcium, including orange juice, sports drinks and breakfast cereals.
The force-clamp technique was applied at maximal calcium activation.
After the calcium load was removed, the rate of the bone remodeling process may reverse [ 40].
Enhanced calcium load might elicit triggered activity (e.g. delayed afterdepolarizations) potentially initiating POAF.
The amount of phosphorus removed increased with calcium loading while the amount removed by charcoal without calcium loading were small.
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